The Andrews model parameters, e.g., maximum specific growth rates (μmax), half saturation, and substrate inhibition constant, were obtained from single-substrate experiments.
Suzuki dt4 fuel mixture series#
The biodegradation kinetics of tetrahydrofuran, benzene (B), toluene (T), and ethylbenzene (E) were systematically investigated individually and as mixtures by a series of aerobic batch degradation experiments initiated by Pseudomonas oleovorans DT4. salarius can also serve as a model for multiple stress tolerance in prokaryotes. salarius is capable of growing at high salt concentration, alkaline pH, hydrocarbon degradation, and also of growth in presence of various metal ions, it can be an attractive candidate for bioremediation of marine oil spills. salarius was found to be capable of weak biofilm formation. To the best of our awareness, this is the first report of catechol metabolism in V. Catechol 2,3 dioxygenase activity in both the organisms was more susceptible to increase in salinity of the growth medium than chlorocatechol 1,2-dioxygenase activity. Effect of salinity of the growth medium on activity of catechol metabolizing enzymes was also studied. salarius, as only this among all the test organisms could grow on the hydrocarbon substrates used, and compared with Pseudomonas oleovorans. Presence of catechol metabolizing enzymes (catechol 2,3 dio-xygenase, chlorocatechol 1,2 dioxygenase, and protocatechuate 3,4 dioxygenase) was checked in V. Hydrocarbon metabolizing capacity of four halotolerant bacteria (Bacillus atrophaeus, Halomonas shengliensis, Halomonas koreensis, and Virgibacillus sala-rius) isolated from saline soil of Khambhat, India was investigated. Hydrocarbon contamination of marine ecosystems has been a major environmental concern. oleovorans DT4 possessed two distinguished benzene pathways. The transient intermediate hydroquinone was detected in benzene biodegradation with THF while catechol in the process without THF, suggesting that P. Additionally, the induction experiments suggested that BTEX and THF degradation was initiated by independent and inducible enzymes.
Conversely, benzene (or toluene) degradation was greatly enhanced by THF leading to a higher degradation rate of 39.68 mg/(h g dry weight) and a shorter complete degradation time about 21 h, possibly because THF acted as an "energy generator". In binary mixtures, THF degradation was delayed by xylene, ethylbenzene, toluene and benzene in descending order of inhibitory effects. Benzene and toluene could be utilized as growth substrates by DT4, whereas cometabolism of m-xylene, p-xylene and ethylbenzene occurred with THF. The efficient tetrahydrofuran (THF)-degrading bacterium, Pseudomonas oleovorans DT4 was used to investigate the substrate interactions during the aerobic biotransformation of THF and BTEX mixtures.
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